| Company | Industry | City |
| TakacsStudios inc. | Brantford | |
| TakeOff CSH | Montr | |
| TalentEgg.ca | Toronto | |
| Tayson Information Technology | Toronto | |
| Technexia | Toronto | |
| Teehan+lax | Toronto | |
| Telefilm Canada | Toronto | |
| Teletoon Canada Inc. | Toronto | |
| TELUS | Ottawa | |
| Ten Plus One Communications | Toronto | |
| Terra Productions | Toronto | |
| ThatMedia Corporation | Professional Services | Toronto |
| The Ant Firm | Professional Services | London |
| The Childrens Group Inc. | Pickering | |
| The Creative Group | Toronto | |
| The Debono Group | Professional Services | Oakville |
| The Fight Network | Television, Broadcasting | Toronto |
| The Herridge Group Inc. | Uxbridge | |
| The Weather Network | Oakville | |
| The Wired Schoolhouse Inc | Toronto | |
| Theatre D Digital Corporation | Toronto | |
| ThinData | Toronto | |
| Tonto Graphics | Toronto | |
| Topix-Mad Dog | Toronto | |
| Toronto Image Works | Toronto | |
| Toronto.com | Toronto | |
| TP Productions | Television Production | Toronto |
| TransGaming Technologies Inc. | Toronto | |
| Trapeze | Toronto | |
| Travesty Productions | Toronto | |
| Tri-Media Integrated Marketing Technologies | Welland | |
| TUSK Interactive | St. Thomas | |
| TV2GO | Toronto | |
| Tvidia Corporation | Kanata | |
| TVOntario | Toronto | |
| Two Solitudes | Toronto |