| Company | Industry | City |
| Sanborg Digital Media Inc. | Mississauga | |
| Sandra Macdonald & Associates | ||
| sarahL.com | Toronto | |
| Scott Thornley + Company | Toronto | |
| Screen Three Media | Mobile | Oakville |
| Senica Canada Ltd. | Thunder Bay | |
| SEZ Social | Toronto | |
| Shaw Street Productions | Toronto | |
| Sheridan College Institute of Technology | Oakville | |
| Sherpa Games Inc. | Games | Markham |
| ShesConnected | Toronto | |
| ShuttleSpace Studio | Toronto | |
| Silicon Knights Inc. | Games | St. Catharines |
| SilverBirch Inc. | Games | Toronto |
| SimEx-Iwerks | Toronto | |
| Simon Conlin | Toronto | |
| Simply Magic Producitons | Toronto | |
| SmallWorld Media Group Inc. | Kingston | |
| Smartculture | Toronto | |
| Smiley Guy Studios | Toronto | |
| Smokebomb Entertainment Inc. | Toronto | |
| Snoek Media | Toronto | |
| Snuffbox Inc. | Toronto | |
| Social Media Group | Dundas/Toronto | |
| Sony Ericsson | Mobile | Research triangle Park |
| Soyabeatz.com | Music Publishing | Mississauga |
| Speedlines Interactive | London | |
| Spin Productions | Toronto | |
| Spongelab Interactive | E-learning and Education | Toronto |
| Spore Productions | Cambridge | |
| St Joseph Media | Toronto | |
| Star Processing | Toronto | |
| Steamworks Media | Toronto | |
| Stephen Leacock Collegiate Institute | Agincourt | |
| Stitch Media | Toronto | |
| Stohn Hay Cafazzo Dembroski Richmond | Toronto | |
| Stohn Hay Cafazzo Dembroski Richmond LLP | Toronto | |
| Story2.OH | Toronto | |
| Strange Duck Media | Toronto | |
| Studio 33b | Toronto |