| Company | Industry | City |
| Nantes Group | Toronto | |
| Navlar | Toronto | |
| NECTAR Foundation | Nepean | |
| Neezo Inc. | Mississauga | |
| Neil Schneider Productions Inc. | Toronto | |
| Nerd Corps Entertainment Inc. | Toronto | |
| NetAccess Systems Inc. | Hamilton | |
| NetPotential Communications Inc. | Toronto | |
| Netstart eBusiness Inc. | St. Catharines | |
| Netvision Communications | Mississauga | |
| New Life Media | Toronto | |
| Nexient Learning Incorporated | Toronto | |
| nGen | Government and Funding Agencies | St. Catharines |
| NI Solutions Group Inc. | Markham | |
| Niagara Enterprise Agency | Unions, Guilds, Associations | Toronto |
| nitch* | Toronto | |
| Nordicity Group Ltd. | Toronto | |
| Norflow | Toronto | |
| Novator Systems Ltd. | Toronto | |
| NTN Buzztime Inc. | Toronto | |
| Nurun Inc. | Toronto |