| Company | Industry | City |
| E-axis.com Inc | Markham | |
| E-Side Design and Communications | Toronto | |
| EA Mobile | Kitchener | |
| Earth Rangers | Woodbridge | |
| ecentricarts | Toronto | |
| Ecko Creative Services | Niagara Falls | |
| EDC (Export Development Canada) | Ottawa | |
| edCetra Training Inc. | Toronto | |
| Edentity Web Systems Inc. | Toronto | |
| EDge Interactive | Toronto | |
| Editors Association of Canada | Toronto | |
| Education Strategy Group | Toronto | |
| Effects-based Analytics | Toronto | |
| Egg Plant Productions Inc. | Toronto | |
| ELB Communications | Toronto | |
| Electric Playground | Toronto | |
| Element K | Toronto | |
| Elemental Inc. | Professional Services | Toronto |
| Elliott Digital Inc | Toronto | |
| ELLIS Entertainment Corporation | Toronto | |
| Emerge2 Digital Inc. | Waterloo | |
| EMI Music Canada | Mississauga | |
| Ent-Law.com - Hall Webber | Toronto | |
| Entertainment Counsel | Toronto | |
| Entertainment Software Association of Canada | Toronto | |
| EqualiTV | Television, Broadcasting | Toronto |
| ESM Group Inc. | Markham | |
| Essential Skills Software | E-learning and Education | Markham |
| Etherlinx E-Business Solutions Inc. | Belleville | |
| Ethnic Channels Group | Television, Broadcasting | Toronto |
| Euforea Inc. | Toronto | |
| ExperiencePoint Inc. | Toronto | |
| eyeon Software Inc. | Toronto | |
| eyeReturn | Toronto |